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Image Search Results
Journal: International Journal of Oncology
Article Title: Downregulation of protein kinase CK2 induces autophagic cell death through modulation of the mTOR and MAPK signaling pathways in human glioblastoma cells
doi: 10.3892/ijo.2012.1635
Figure Lengend Snippet: Downregulation of protein kinase CK2 affects the mTOR and ERK1/2 signaling pathways. Cell lysates (30 μ g) from cells treated as indicated in the figure were analyzed by western blot analysis using antibodies against the indicated proteins or their phosphorylated form. Anti-β-actin was applied to confirm equal protein loading. At least 4 separate experiments were performed obtaining similar results. Data from one representative experiment are shown.
Article Snippet: The primary antibodies employed in this study were: mouse monoclonal anti-CK2β and -CK2α (both from Calbiochem); rabbit polyclonal anti-CK2α′ obtained by immunizing rabbits with a specific peptide (SQPCADNAVLSSGTAAR) deriving from human CK2α′; mouse monoclonal anti-β-actin (Sigma);
Techniques: Western Blot
Journal: International Journal of Oncology
Article Title: Downregulation of protein kinase CK2 induces autophagic cell death through modulation of the mTOR and MAPK signaling pathways in human glioblastoma cells
doi: 10.3892/ijo.2012.1635
Figure Lengend Snippet: Model of the proposed influence of CK2 on autophagy induction. The model suggests the placement and function of CK2 with respect to the major signaling cascades regulating autophagy. CK2 has been reported to be a master regulator of cellular functions in virtue of its ability to play a ‘lateral means’ of pathways intervention . Here, we show that effective CK2-mediated autophagy induction is achieved by the simultaneous targeting of the ERK1/2-and mTOR signaling pathways. Additional details are reported in the text.
Article Snippet: The primary antibodies employed in this study were: mouse monoclonal anti-CK2β and -CK2α (both from Calbiochem); rabbit polyclonal anti-CK2α′ obtained by immunizing rabbits with a specific peptide (SQPCADNAVLSSGTAAR) deriving from human CK2α′; mouse monoclonal anti-β-actin (Sigma);
Techniques: